Determination of 7 flavonol glycosides in Ginkgo biloba reference extract.
- Author:
Jing-hui WANG
;
Jing CHEN
;
Meng-meng WANG
;
Xin-tong FU
;
You-gen CHEN
;
Hong-zhu GUO
- Publication Type:Journal Article
- MeSH:
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Flavonols;
chemistry;
isolation & purification;
Ginkgo biloba;
chemistry;
Glucosides;
chemistry;
isolation & purification;
Molecular Structure;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2015;40(20):4018-4021
- CountryChina
- Language:Chinese
-
Abstract:
Six flavonol glycosides were isolated and calibrated from Ginkgo biloba extract, and then used to calibrate the content in 2 baiches of G. biloba reference extract, so was rutin. RSD values of rutin, kaempferol-3-O-rutinoside, kaempferol-3-O-rhamnoside-2-glu- coside, quercetin-3-O-rhamnop-yranosyl-2-O-(6-O-p-coumaroyl)-glucoside, kaempferol-3-O-rhamnopyranosyl-2-O-(6-O-p-coum-aroyl) - glucoside were around 1.1%-4.6%, nevertheless, RSD values of quercetin-3-O-glucoside and isorhamnetin-3-O-rutinoside were more than 5%. According to the results, the reference extract of G. biloba can be used as the substitute to determine rutin, kaempferol-3-O- rutinoside, kaempferol-3-O-rhamnoside-2-glucoside, quercetin-3-O-rhamnopyranosyl-2-O-(6-O-p-coumaroyl)-glucoside and kaempferol-3-0-rhamnopyranosyl-2-O-(6-O-p-coumaroyl)-glucoside instead of corresponding reference substances. So reference extract in place of single component reference in assay is feasible.
