HPLC simultaneous determination of contents of 5 saponin constituents in Ophiopogonis Radix.
- Author:
Fa-ming WU
;
Xiao-yang CAI
;
Pan WANG
;
Xiao-hong BAO
;
Min LI
;
Juan ZHOU
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Ophiopogon;
chemistry;
Plant Roots;
chemistry;
Saponins;
chemistry;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2015;40(20):4022-4025
- CountryChina
- Language:Chinese
-
Abstract:
This research is to establish an HPLC method for the simultaneous determination of ophiopogonin D, ophiopogonin D', ophiopogonin C, deacetylophiopojaponin A and ophiogenin-3-O-α-L-rhamnosyl-(1-->2)-β-D-glucoside in Ophiopogonis Radix. HPLC-ELSD analysis was performed on a Kromasil 100-5 C₁₈ column (4.6 mm x 250 mm, 5 µm), with the mobile phase of acetonitrile (A) -water (B) in gradient elution mode (0-45 min, 35%-55% A), at a flow rate of 1 mL · min⁻¹. The column temperature was 35 °C and the drift tube temperature was 100 °C in a gas flow rate of 3.0 L · min⁻¹. The result showed that baseline of all the 5 constituents was well separated, and every constituent had wide linearity range and good linear relation (r > 0.999). The recovery rate was between 95.75% and 103.1%. The new established method for simultaneous determination of saponin constituents in Ophiopogonis Radix was sensitive and has good, repeatability. It could be applied to quality evaluation of Ophiopogonis Radix.
