Effect of pulchinenoside on FZD8 expression of adjuvant arthritis rats.
- Author:
Cheng-gui MIAO
;
Guo-liang ZHOU
;
Mei-song QIN
;
Jian-zhong CHEN
;
Cheng-feng LI
;
Hua-qi HE
;
Bing ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Arthritis, Experimental;
drug therapy;
genetics;
metabolism;
Disease Models, Animal;
Drugs, Chinese Herbal;
administration & dosage;
Humans;
Male;
Rats;
Rats, Sprague-Dawley;
Receptors, Cell Surface;
genetics;
metabolism;
Saponins;
administration & dosage
- From:
China Journal of Chinese Materia Medica
2015;40(20):4063-4067
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of pulchinenoside (PULC) on the Frizzled (FZD) expression of adjuvant arthritis ( AA) rats. AA rats were prepared through the toe injection with complete Freund's adjuvant to culture fibroblast-like synoviocytes (FLS). The effect of the oral administration with PULC on the FZD8 expression was detected by the real time qPCR. The effect of FZD8 knockout on the expressions of IL-1, IL-6, IL-8 were detected by MTT and ELISA. The role of miR-375 in the abnomal expression of FZD8 was detected by the real time qPCR. The results showed signfiicant decrease in the FZD8 expression among AA rats, FLS proliferation ater FZD8 knockout and IL-1, IL-6, IL-8 expressions and notable increase in miR-375 expression after the oral administration with PULC. The up-regulated miR-375 expression can inhibit the FZD8 expression. PULC may inhibit the FZD8 expression by up-regulating the miR-375 expression.
