Effects of combination of glycyrrhizin acid, ligustrazine and puerarin on LPS-induced cytokines expression in macrophage.
- Author:
Zhao LIU
;
Ju-ying ZHONG
;
Er-ning GAO
;
Hong YANG
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents;
pharmacology;
Cytokines;
Glycyrrhizic Acid;
pharmacology;
Isoflavones;
pharmacology;
Lipopolysaccharides;
immunology;
Macrophages;
drug effects;
immunology;
Mice;
NF-kappa B;
genetics;
immunology;
Pyrazines;
pharmacology;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2015;40(20):4068-4074
- CountryChina
- Language:Chinese
-
Abstract:
To study the anti-inflammatory activity of glycyrrhizin acid, ligustrazine and puerarin. In the study, the liquichip-based high-throughput synchronous detection technique for 23 inflammatory factors, uniform design, comprehensive weight method were adopted to study the effect of different combined administration of glycyrrhizin acid, ligustrazine and puerarin in inhibiting the expression of lipopolysaccharide (LPS)-induced RAW264. 7 cells and multiple inflammatory cytokines. In the study, the uniform design table U₉ (9³) was adopted to design doses of glycyrrhizin acid, ligustrazine and puerarin. The liquichip technique was used to detect the effect of different combined administration of glycyrrhizin acid, ligustrazine and puerarin on the 23 cytokines expressed in LPS-induced mouse macrophage RAW264. 7 inflammation model. The traditional Chinese medicine component optimization software and the improved least angle regression algorithm were used to analyze the dose-effect relationship among the three components and the cytokine inhibition rate and produce the regression equation. The comprehensive weight method was applied to get the optimal dose ratio of glycyrrhizic acid, ligustrazine and puerarin with highest efficacy of 25:2:13 and verify the optimal dose ratio. The verification results were consistent with the prediction trend, indicating the accuracy of the mathematical model for predicting the experiment. The experimental results showed the multi-target and multi-level efficacies of glycyrrhizic acid, ligustrazine and puerarin and the high anti-inflammatory activity of their combined administration, which provides powerful basis for subsequent drug development.
