Cloning and expression analysis of 4- (cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase gene in Tripterygium wilfordii.
- Author:
Yu-ru TONG
;
Ping SU
;
Yu-jun ZHAO
;
Meng ZHANG
;
Xiu-juan WANG
;
Tian-yuan HU
;
Wei GAO
;
Lu-qi HUANG
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Cloning, Molecular;
Computational Biology;
Gene Expression Regulation, Plant;
Models, Molecular;
Molecular Sequence Data;
Phosphotransferases (Alcohol Group Acceptor);
chemistry;
genetics;
metabolism;
Phylogeny;
Plant Proteins;
chemistry;
genetics;
metabolism;
Sequence Alignment;
Tripterygium;
chemistry;
enzymology;
genetics
- From:
China Journal of Chinese Materia Medica
2015;40(21):4165-4170
- CountryChina
- Language:Chinese
-
Abstract:
4-(Cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase is a key enzyme in the biosynthesis pathway of terpenoids. According to the transcriptome database, the specific primers were designed and used in PCR. The bioinformatic analysis of the sequenced TwCMK gene was performed in several bioinformatics software. The Real-time fluorescence quantification polymerase chain reaction (RT-qPCR) were used to detect the expression levels of TwCMK from T. wilfordii after elicitor MeJA supplied. The results showed that the full length of TwCMK cDNA was 1 732 bp encoding 387 amino acids. The theoretical isoelectric point of the putative TwCMK protein was 5.79 and the molecular weight was about 42.85 kDa. MeJA stimulated the rising of TwCMK expression in suspension cell and signally impacted at 24 h. The research provides a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis.
