Preparation and anti-cancer activity in vitro of curcumin loaded mesoporous silica nanoparticle.
- Author:
Li-li HE
;
Jian GU
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
chemistry;
pharmacology;
Curcumin;
chemistry;
pharmacology;
Drug Carriers;
chemistry;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Humans;
Nanoparticles;
chemistry;
Neoplasms;
drug therapy;
Particle Size;
Porosity;
Silicon Dioxide;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(21):4189-4193
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to prepare curcumin (Cur) loaded mesoporous silica nanoparticle (Cur-MSN), evaluate its release behavior and anti-cancer activity in vitro. Mesoporous silica nanoparticle (MSN) was prepared by polymerization method and Cur-MSN was obtained using solvent evaporation method and impregnation centrifugation method. The preparation method was optimized using entrapment efficiency (EE) and loading efficiency (LE) as indexes. Cur-MSN was characterized with scanning electron microscope and its particle size and zeta potential were determined. Finally, in vitro release behavior in 0.2% SDS solution and its cell-killing effect on HeLa cells were also evaluated. The Cur-MSN prepared with process optimization method was round and uniform and exhibited typical mesoporous characterization. The mean particle size and Zeta potential of Cur-MSN were 75.8 nm and -30.1 mV, respectively. EE and LE of three batches of Cur-MSN were (72.55 ± 2.01)% and (16.21 ± 1.12)%, respectively. In vitro release behavior of Cur-MSN showed a sustained release profile with 83.5% cumulative release within 96 h. The killing effect of Cur-MSN on HeLa cells was dose-dependent with IC50 of 19.40 mg x L(-1), which was similar to that of Cur.
