Constituents with anti-oxidative activity from seeds of Jufeng grape.
- Author:
An-jun DENG
;
Hai-jing ZHANG
;
Zhi-hui ZHANG
;
Zhi-hong LI
;
Lin MA
;
Feng WU
;
Lian-qiu WU
;
Wen-jie WANG
;
Hai-lin QIN
- Publication Type:Journal Article
- MeSH:
Antioxidants;
chemistry;
isolation & purification;
Magnetic Resonance Spectroscopy;
Plant Extracts;
chemistry;
isolation & purification;
Seeds;
chemistry;
Vitis;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(21):4208-4211
- CountryChina
- Language:Chinese
-
Abstract:
Taking application of some isolation and purification technologies, including crushing, solvent extraction, preliminary solvent isolation, column chromatographies over silica gel and Sephadex LH-20 gel and preparative HPLC, 8 compounds were obtained from the seeds of Jufeng grape sourced from market. Their structures were identified by spectroscopic methods and comparison with literature values as Catechin (1), Epicatechin (2), quercetin (3), ethylgallate (4), rel-(2S, 3R) -2-(4-hydroxy-3-methoxyphenyl) -3- (hydroxymethyl)-5-(3-hydroxypropyl)-2,3-dihydrobenzofuran-7-ol (5), rel-(2α, 3β)-7-O-methylcedrusin (6), rel-(1R,2S)-1-(4-hydroxy-3-methoxyphenyl) -2-(4-(3-hydroxypropyl) -2-methoxyphenoxy) propane-1,3-diol (7), and (+) -isolariciresinol (8), respectively. Compounds 5-8 were serial lignans isolated from the seeds of grape for the first time. Structurally, 5 and 6 belong in benzofuran-8,3'-neolignans, 7 in 8,4'-oxyneolignan, and 8 in 8,8' :2,7'-cyclolignan. According to in vitro activity evaluation conducted in cell model, compound 6 showed significant anti-oxidative ability, with the activity of RAW264. 7 cell superoxide dismutase being raised evidently in the test as compared with the positive anti-oxidative agents, compounds 1 and 2.
