Design space approach to optimize first ethanol precipitation process of Dangshen.
- Author:
Zhi-lin XU
;
Wen-hua HUANG
;
Xing-chu GONG
;
Tian-tian YE
;
Hai-bin QU
;
Yan-gang SONG
;
Dong-lai HU
;
Guo-xiang WANG
- Publication Type:Journal Article
- MeSH:
Chemical Precipitation;
Chemistry, Pharmaceutical;
methods;
Codonopsis;
chemistry;
Drugs, Chinese Herbal;
chemistry;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2015;40(22):4411-4416
- CountryChina
- Language:Chinese
-
Abstract:
Design space approach is applied in this study to enhance the robustness of first ethanol precipitation process of Codonopsis Radix (Dangshen) by optimizing parameters. Total flavonoid recovery, dry matter removal, and pigment removal were defined as the process critical quality attributes (CQAs). Plackett-Burman designed experiments were carried out to find the critical process parameters (CPPs). Dry matter content of concentrated extract (DMCE), mass ratio of ethanol to concentrated extract (E/C ratio) and concentration of ethanol (CEA) were identified as the CPPs. Box-Behnken designed experiments were performed to establish the quantitative models between CPPs and CQAs. Probability based design space was obtained and verified using Monte-Carlo simulation method. According to the verification results, the robustness of first ethanol precipitation process of Dangshen can be guaranteed by operating within the design space parameters. Recommended normal operation space are as follows: dry matter content of concentrated extract of 45.0% - 48.0%, E/C ratio of 2.48-2.80 g x g(-1), and the concentration of ethanol of 92.0% - 92.7%.
