Effect of shenwu capsule on the peripheral blood lymphocyte DNA of rat model induced by beta-amyloid injection.
- Author:
Hui WANG
1
;
Lin LI
;
Cui-fei YE
;
Lan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; Animals; Capsules; DNA Damage; drug effects; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Lymphocytes; drug effects; Male; Neurodegenerative Diseases; chemically induced; pathology; Neuroprotective Agents; administration & dosage; pharmacology; Panax; chemistry; Plants, Medicinal; chemistry; Polygonum; chemistry; Pueraria; chemistry; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2004;29(2):176-179
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of the new Traditional Chinese Compound Shenwu Capsule on the damage of lymphocyte DNA and lipid peroxidation in peripheral blood of rats induced by beta-amyloid injection.
METHODThe animal model was made by injection of beta-amyloid25-35 into hippocampus of rats. DNA damage of lymphocytes was measured by the single cell gel electrophoresis (SCGE) combined with the laser scanning confocal microscopy (LSCM). The content of malondialdehyde (MDA) was determined by TBA assay.
RESULTShenwu Capsule decreased the rate of the comet-like cell, comet-like cell lengh (TCL), and tail moment (TM) of lymphocytes in peripheral blood of model rats. Shenwu Capsule also declined the MDA content in serum of Abeta model rats.
CONCLUSIONShenwu Capsule has protective effect on peripheral blood lymphocyte DNA.
