A study on quality standard for Herba Siegesbeckidae.
- Author:
Zhi-hong CHENG
1
;
Gui-xin CHOU
;
Zheng-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Asteraceae; chemistry; Drugs, Chinese Herbal; analysis; Pharmacognosy; standards; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(4):257-259
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the qualitative and quantitative methods of Herba Siegesbeckiae.
METHODA TLC method was used for qualitative identification and a HPLC analysis was applied for quantitative determination of Herba Siegesbeckiae with kirenol as the reference substances.
RESULTChloroform-methanol-formic acid (25:5:1) as a mobile phase of TLC, the spot of kirenol can be easily detected; Methanol extracts of Herba Siegebeckiae were separated on a Polaris C18 column with acetonitrile-water (25:75) as mobile phase and kirenol was separated well. The average content of kirenol in Herba Siegebeckiae was 0.14%. A good linear relationship between the peak areas and injected amounts of kirenol in the range of 0.19-14.9 microg and the average recovery was 100.0% (RSD = 2.4%).
CONCLUSIONThe method can be used for qualitative identification and quantitation determination of Herba Siegesbeckiae.
