Determination of triptolide in Tripterygium total terpenoids tablets by HPLC.
- Author:
Cun-hai PU
1
;
Ming-fang HE
;
Xiao-qian ZHANAG
;
Jia SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Diterpenes; analysis; Epoxy Compounds; Phenanthrenes; analysis; Plants, Medicinal; chemistry; Quality Control; Tablets; Terpenes; administration & dosage; chemistry; isolation & purification; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(4):266-267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.
METHODA Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.
RESULTThe linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.
CONCLUSIONThe method is simple, rapid and accurate and can be used for quality control of the tablets.
