OBJECTIVEThe fluorescence property of sophoramine was studied and a spectrofluorimetric method was established to determine the sophoramine content.

METHODIn 20% ethanol solution, with excitation wavelength at 394 nm and emission wavelength at 467 nm, the fluorescence intensity of sophoramine can be detected by the fluorophotometer.

RESULTSophoramine content can be determined with external standard method by fluorophotometer. The linear relationship between fluorescence intensity and concentration is kept in the range of 10-200 microg x mL(-1). The regression equation is Int = 1.137C + 3.875. The recovery rate is 98%-102%.

CONCLUSIONUtilizing the fluorescence character of sophoramine, its content can be determined fast and sensitively. The analysis is not interfered by the existing matrine and oxymatrine. The method has high selectivity and the results is satisfying.