Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte.
- Author:
Wei GU
1
;
Wen-heng ZENG
;
Hai-ying HU
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; metabolism; Adipocytes; cytology; metabolism; Adiponectin; Animals; Berberine; pharmacology; Insulin; pharmacology; Intercellular Signaling Peptides and Proteins; biosynthesis; genetics; Mice; RNA, Messenger; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2005;30(4):286-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte.
METHODThe 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference.
RESULTThe level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05).
CONCLUSIONIn vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.
