Effects of Epimedium pubescens icariine on proliferation and differentiation of human osteoblasts.
- Author:
Xiao-xue YIN
1
;
Zhong-qiang CHEN
;
Geng-ting DANG
;
Qing-jun MA
;
Zhong-jun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; biosynthesis; genetics; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Epimedium; chemistry; Flavonoids; isolation & purification; pharmacology; Humans; Osteoblasts; cytology; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Transforming Growth Factor beta; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2005;30(4):289-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of epimedium pubescens icariine on the proliferation and differentiation of human osteoblasts.
METHODHuman osteoblasts were obtained by inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR.
RESULTEpimedium pubescens icariine at the dose of 20 microg x mL(-1) increased greatly the proliferation and differentiation of human osteoblasts and promoted the expression of BMP-2 mRNA.
CONCLUSIONEpimedium pubescens icariine enhances significantly the proliferation and differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.
