Studies on the anticancer effects of total alkaloid from Viscum coloratum.
- Author:
Hai-yan PENG
1
;
Yong-hong ZHANG
;
Ying HAN
;
Meng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; isolation & purification; pharmacology; Animals; Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Breast Neoplasms; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Esophageal Neoplasms; pathology; Female; Humans; Liver Neoplasms, Experimental; drug therapy; pathology; Mice; Neoplasm Transplantation; Plants, Medicinal; chemistry; Viscum; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(5):381-387
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anticancer effects of total alkaloid from Viscum coloratum in vivo and vitro.
METHODIn vitro, MTT assays were used t o measure the inhibitory effect. Cells at period of logarithmic growth were incubated for 24 hours. Then total alkaloid of various concentrations were added. 24 hours later, supernatant was removed and MTT was added. 4 hours after that, DMSO was added, then 30 minutes later, A value was measured. In vivo, suspension of carcinoma cells was implanted in the mice's limbs subcutaneously, 0.2mL each. 24 hours later, the mice were grouped randomly. Fed by total alkaloid continuously for 7 days, the mice were sacrificed. The tumors were weighed and calculated the inhibitory rates.
RESULTIn vitro, it shows that total alkaloid has prominent inhibitory effect on the growth of carcinoma cells. In vivo, it shows that total alkaloid can inhibit the growth of tumors and prolong the survival days of the mice bearing tumors.
CONCLUSIONTotal alkaloid from Viscum coloratum has the activities of anticancer.