The study on the treatment of Oviducts Ranae protein with enzyme hydrolysis.
- Author:
Jin-Ming LI
1
Author Information
1. Harbin Business University, Harbin 150040, China. sunximi@yeah.net
- Publication Type:Journal Article
- MeSH:
Amphibian Proteins;
chemistry;
isolation & purification;
Animals;
Estradiol;
analysis;
Female;
Hydrogen-Ion Concentration;
Hydrolysis;
Materia Medica;
chemistry;
Oviducts;
chemistry;
Papain;
chemistry;
Pepsin A;
chemistry;
Rana esculenta
- From:
China Journal of Chinese Materia Medica
2005;30(6):430-432
- CountryChina
- Language:Chinese
-
Abstract:
The paper introduced a process to enhance the yield of Oviducts Ranae protein using in vitro enzyme hydrolysis. The treatment process included two steps: (1) a 3 - 4 h of hydrolysis of a 0.025 g x g(-1) concentration of substance, at pH 7 and 60 degrees C, using 4% of papain; and (2) followed with a 6 - 8 h hydrolysis, at pH 2 - 2.5 and 60 degrees C, using 3% of pepsin. This treatment process significantly improved the lyophilized Oviducts Ranae in solubility and fluidity, which is convenient for the relative pharmaceutical preparations.
