Study on RP-HPLC fingerprint of compound hongcao injection.
- Author:
Yan-Yu LAN
1
;
Li-Na LIU
;
Yong-Lin WANG
;
Yong-Jun LI
;
Yong HUANG
;
Xun HE
;
Lin ZHENG
;
Ai-Min WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; Plants, Medicinal; chemistry; Polygonum; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(6):440-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the fingerprint detecting standard of Compound Hongcao Injection by reversed phase high performance liquid chromatography.
METHODThe chromatographic conditions were as follows: an Inersil-ODS-3 column was used; the mobile phases (acetontrile and 0.1% phosphoric acid) with gradient elution; the flow rate 1.0 mL x min(-1) and the UV absorbance detection at 300 nm.
RESULTUnder the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation according to the technical requirements of fingerprint on Injection of Chinese traditional medicine.
CONCLUSION17 common peaks as well as their retention times and peak area ratios on HPLC fingerprint can be used as the important parameters of the quality control for Compound Hongcao Injection.
