Discovery of the main active constituents of siwu mixture for reversing the multidrug resistance of human erythrocyte leukemic cell line K562/ADM.
- Author:
Peng SHEN
1
;
Lei XIA
;
Hai-jiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Doxorubicin; Drug Combinations; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Drugs, Chinese Herbal; chemistry; isolation & purification; pharmacology; Humans; K562 Cells; Ligusticum; chemistry; Plants, Medicinal; chemistry; Polysaccharides; isolation & purification; pharmacology; Rehmannia; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(7):520-523
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the main active constituents of Siwu mixture for reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM.
METHODMacroporous resin was used to separate Siwu mixture and an in vitro model was used to evaluate the reversal index of each fraction for discovery of active fraction. HPLC-MS was used for investigating the main active constituents of Siwu mixture.
RESULTThe reversal index of the gross polysaccharide of Rehmannia glutinosa was significantly higher than that of blank control (P < 0.01). There was little difference between the reversal effect of the gross polysaccharide of Rehmannia glutinosa and that of the active fraction A (P > 0.05).
CONCLUSIONThe gross polysaccharide of Rehmannia glutinosa is one of the main active constituents of Siwu mixture.
