Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC.
- Author:
Fang-li WU
1
;
Ai-zhen LI
;
Hui-fang MAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Galantamine; analysis; Lycoris; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(7):523-525
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determination of galanthamine in Lycoris radiata.
METHODHPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.
RESULTGalanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).
CONCLUSIONThe method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.