The dynamic effect of yiqi qingwen jiedu heji on the protein expressions of cytokine IFN-gamma, TNF-alpha, IL-10 and IL-6 in the lungs of mice infected by IV FM1.
- Author:
Cheng-xiang WANG
1
;
Gui-xin GAO
;
Shou-chao WEI
;
Hong-ri XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytokines; metabolism; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Interferon-gamma; metabolism; Interleukin-10; metabolism; Interleukin-6; metabolism; Lung; metabolism; Male; Mice; Mice, Inbred BALB C; Orthomyxoviridae; Orthomyxoviridae Infections; metabolism; virology; Plants, Medicinal; chemistry; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(7):541-544
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find the function and functioning mechanism of Yiqi Qingwen Jiedu Heji in resisting influenza immune damage by studying its effect on the expressions of cytokine.
METHODTaking IV FM1 infected mice as its model and doing ELISA (double antibody sandwichenzyme linked immunosorbent assay), we dynamically observed the change of cytokine TNF-alpha, IL-6, IFN-gamma and IL-10 after giving Yiqi Qingwen Jiedu Heji treatment.
RESULT AND CONCLUSIONAfter the mice are infected by influenza virus, their protein expressions of the model group are higher than those of the control group, of which TNF-alpha, IL-6, IFN-gamma reach the peak in three days. The three expressions of Yiqi Qingwen Jiedu Heji treated group are decreased and the decrease becomes remarkable on the third day, compared with those of the model group. However, the expression of IL-10 of the treated group is remarkably increased. It indicates that Yiqi Qingwen Jiedu Heji can resist the expressions of TNF-alpha, IL-6 and IFN-gamma pro-inflammatory cytokine,increase the expression of IL-10, and thus, alleviate inflammatory injury. So the clinical application of such medicine can shorten the course of disease.
