Ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen.
- Author:
Cai LI
1
;
Xiang-jun LI
;
Chun-sheng MIAO
;
Xiu-yun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen; metabolism; Diabetes Mellitus, Experimental; metabolism; Female; Glycopeptides; isolation & purification; pharmacology; Male; Panax; chemistry; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Solubility; Tail; Tendons; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(7):544-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the ameliorative effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen.
METHODELISA was used to determine the inhibitory effect of ginseng glycopeptide on cross-linking of rat tail tendon collagen in vitro. After ginseng glycopeptide was intraperitoneally administrated to streptozotocin-induced diabetic rats for 12 weeks, the acid solubility, limited pepsin degradation properties and solubility in SDS-2-mercaptoethanol of the rat tail tendon collagen were determined, and the effect of ginseng glycopeptide on the tail tendon collagen cross-linking was evaluated.
RESULTGinseng glycopeptide inhibited significantly the cross-linking of rat tail tendon collagen in vitro. The solubility of the tail tendon collagen (in acid, pepsin and SDS-2-mercaptoethanol) was markedly decreased in diabetic rats and ginseng glycopeptide-treated diabetic rats had significantly an increase in the collagen solubility in the above-mentioned solutions, suggesting that ginseng glycopeptide decreased severity of the collagen cross-linking.
CONCLUSIONGinsengglycopeptide exhibits an significantly ameliorative effect on cross-linking of rat tail tendon collagen.
