Effects of different factors on direct induction of microtubers from explants in Pinellia ternate.
- Author:
Ai-Min ZHANG
1
;
Sheng-Yu YANG
;
Jian-Ping XUE
;
Wei SHENG
;
Xiang GAO
;
Yan-Fang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Carbon; pharmacology; Caseins; pharmacology; Culture Media; Culture Techniques; Glucose; pharmacology; Pinellia; growth & development; Plant Growth Regulators; pharmacology; Plant Leaves; growth & development; Plant Tubers; growth & development; Plants, Medicinal; growth & development; Polyethylene Glycols; pharmacology; Sucrose; pharmacology
- From: China Journal of Chinese Materia Medica 2005;30(8):576-579
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose.
METHODUsing the orthogonal design method.
RESULT AND CONCLUSIONThe optimal media to directly induce microtubers from leaves were MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3% and MS+ 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + sucrose 3%. Optimal media to directly induce microtubers from tubers were MS + 6-BA 1.0 mg x L(-1) + PEG 5% + sucrose 5% and MS+ CH 500 mg x L(-1) + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + AC 0.5% + sucrose 5%. Media suitable for plantlet growth were MS + 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + AC 0.5% + sucrose 5% and MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3%.
