Determination of flavonoids in buds of Herba Artemisiae Scopariae by HPLC.
- Author:
Sheng LIN
1
;
Qi-Wei ZHANG
;
Ning-Ning ZHANG
;
Yong-Xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Artemisia; chemistry; Chromatography, High Pressure Liquid; Flavones; analysis; Flowers; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(8):591-594
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae.
METHODThe sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution (2.5:21.5:76) and detection wavelength was 347 nm.
RESULTThe arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good (r = 0.9992, 0.9999, 0.9997, 0.9997) in ranges of 0.035-0.56, 0.043-0.69, 0.040-0.64 and 0.040-0.64 microg, respectively. The average recoveries were 96.8%, 97.3%, 96.8%, 98.5% and RSD the values of repeatability were 2.5%, 3.0%, 1.5%, 3.2%, respectively.
CONCLUSIONThe method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.
