Determination of complanatoside A in semen Astragali complanati by HPLC.
- Author:
Jian-Jun ZHANG
1
;
Xing-Li YAN
;
Yu-Jie ZHANG
;
Yu-Ping WANG
;
Qiu-Ju ZHANG
;
Feng-Ying ZENG
;
Wei LI
;
Li-Na OU
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus Plant; chemistry; Chromatography, High Pressure Liquid; methods; Flavonols; analysis; Glucosides; analysis; Plants, Medicinal; chemistry; Quality Control; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(8):600-602
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the determination method for complanatoside A in seeds of Astragalus complanatus.
METHODAn HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.
RESULTThe good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.
CONCLUSIONThe established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.
