Roles of follicular helper T cells and follicular regulatory T cells in pathogenesis of Henoch-Schönlein purpura in children.
- Author:
Chun-Mei WANG
1
;
Yuan LUO
;
Ying-Chao WANG
;
Guang-Yao SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; DNA-Binding Proteins; genetics; Female; Humans; Male; Programmed Cell Death 1 Receptor; genetics; Proto-Oncogene Proteins c-bcl-6; Proto-Oncogene Proteins c-maf; genetics; Purpura, Schoenlein-Henoch; etiology; immunology; T-Lymphocytes, Helper-Inducer; immunology; T-Lymphocytes, Regulatory; immunology
- From: Chinese Journal of Contemporary Pediatrics 2015;17(10):1084-1087
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the roles of follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells in the pathogenesis of Henoch-Schönlein purpura (HSP) in children.
METHODSPeripheral blood samples were collected from 40 HSP children and 25 healthy controls. The percentages of Tfh and Tfr cells were measured by flow cytometry; the mRNA expression levels of Bcl-6, c-MAF, Blimp-1, and PD-1 in peripheral blood were measured by real-time polymerase chain reaction.
RESULTSCompared with the controls, the children with HSP had significantly increased percentage of Tfh cells and Tfh/Tfr ratio but a significantly reduced percentage of Tfr cells in the peripheral blood (P<0.05). Compared with the controls, the children with HSP had significantly increased mRNA expression of Bcl-6 and c-MAF but significantly reduced mRNA expression of Blimp-1 in CD4+ T cells (P<0.05), and had significantly increased mRNA expression of PD-1 but significantly reduced mRNA expression of Blimp-1 in CD4+CD25+ regulatory T cells (P<0.05).
CONCLUSIONSAbnormal percentages of Tfh and Tfr cells may be involved in the pathogenesis of HSP in children, and over-expression of Bcl-6, c-MAF, and PD-1 mRNA and inhibited expression of Blimp-1 mRNA may be considered as important reasons for abnormal percentages of Tfh and Tfr cells.
