Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction.
- Author:
Yan WANG
1
;
Xiaoyuan XU
;
Wei HE
;
Zhihong LIU
;
Weibo GONG
;
Qinhuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Viral; blood; Fluorescent Dyes; Hepatitis B virus; genetics; Humans; Organic Chemicals; Polymerase Chain Reaction; methods; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(2):160-161
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.
METHODSQuantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.
RESULTSTaqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.
CONCLUSIONSSybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.
