Construction and expression of recombinant retrovirus vector carrying HBV vector.
- Author:
Dianxing SUN
1
;
Darong HU
;
Guanghui WU
;
Xueling HU
;
Juan LI
;
Gongren FAN
Author Information
- Publication Type:Journal Article
- MeSH: Genetic Therapy; Genetic Vectors; Hepatitis B Core Antigens; biosynthesis; Hepatitis B virus; genetics; Humans; Recombination, Genetic; Retroviridae; genetics; Tumor Cells, Cultured; Virus Replication
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(2):162-165
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo explore the possibility of using retrovirus vector to carry HBV vector, and to prove that replication defective HBV could be normally packaged.
METHODSTwo kinds of full length of mutant HBV gene, which express dominant negative mutants, were inserted into retrovirus vector. After recombinant retroviruses were harvested, they were used to infect Hep G2 and 2.2.15 cell line. Then the expression of HBV core antigen in the Hep G2 cell was examined by immune fluorescence, and the existence of recombinant HB virion in the culture medium was examined by PCR.
RESULTSHigh titer of recombinant retroviruses were obtained in the culture medium of transfected PA317 cell line. Core antigen was detectable in the recombinant retrovirus infected Hep G2 cell. Recombinant HB virion was detectable in the culture medium of recombinant retrovirus infected 2.2.15 cell.
CONCLUSIONSThe results suggested that recombinant retrovirus could carry HBV vector and express HBV products. When structural protein is offered by wt-HBV, the recombinant retrovirus may function as HBV vector, therefore it could be used in anti?HBV gene therapy.
