Cloning and expression of simplex herpes virus ? US4 fragment.
- Author:
Yao YI
1
;
Wenbo XU
;
Mingcheng ZHANG
;
Yongdong ZHOU
;
Yong LI
;
Shengli BI
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Viral; immunology; Cloning, Molecular; Gene Expression; Herpesvirus 2, Human; genetics; Humans; Immunoglobulin G; blood; Polymerase Chain Reaction; Recombinant Proteins; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(2):168-170
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo get early laboratory study of type specific antigenicity of herpes simplex virus II.
METHODSPCR and prokaryotic expression technique.
RESULTSHerpes simplex virus II type specific gene fragment was expressed in E.coli and the products can be used as specific antigen for the detection of anti\HSV in the recovery sera.
CONCLUSIONSCloning and express of HSVII type specific antigen found the basis for developing specific diagnosis methods and vaccine of HSV.
