Expression, purification and diagnostic application of EBV TK kinase.
- Author:
Bin HUANG
1
;
Bojun LI
;
Weimin ZHOU
;
Guohua PI
;
Weiping TANG
;
Shuyan GU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; methods; Herpesvirus 4, Human; enzymology; Humans; Immunoglobulin G; blood; Nasopharyngeal Neoplasms; diagnosis; Recombinant Proteins; biosynthesis; immunology; isolation & purification; Sensitivity and Specificity; Thymidine Kinase; biosynthesis; isolation & purification
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(2):184-186
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo find a rapid and sensitive method for early diagnosis of nasopharyngeal carcinoma by using EBV TK kinase.
METHODSProkaryotic expression plasmid pRSETTK was constructed. EBV TK kinase was highly expressed in E.coil BL21 (DE3). The authors identified specificity of TK kinase by Western blot, then used purified TK kinase in ELISA to detect the IgG antibody in the serum of NPC patients.
RESULTSSpecific IgG antibody against TK kinase was found in the serum of NPC patients. The specificity and sensitivity of TK kinase were both 100% in Western blot and were 98.0% and 93.4% respectively in ELISA.
CONCLUSIONSThe EBV TK kinase showed high specificity and sensitivity in ELISA, therefore it can be used for early diagnosis of NPC
