DNA methylation-mediated epigenetic silencing of miR-720 contributes to leukemogenesis in acute myeloid leukemia.
- VernacularTitle:DNA甲基化介导的miR-720沉默与急性髓系白血病生物学特征的关系
- Author:
Shanhao TANG
1
;
Renzhi PEI
1
;
Kongfei LI
1
;
Junxia MA
1
;
Peisheng ZHANG
1
;
Ying LU
1
;
Xuhui LIU
1
;
Xiaohong DU
1
;
Dong CHEN
1
;
Keya SHA
1
;
Junjie CAO
1
;
Shuangyue LI
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; DNA Methylation; Epigenesis, Genetic; Humans; Leukemia, Myeloid, Acute; genetics; pathology; MicroRNAs; genetics; Promoter Regions, Genetic
- From: Chinese Journal of Hematology 2014;35(11):1009-1012
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression level and regulation mechanism of miR-720 as well as the association of miR-720 expression with leukemia biological characteristics.
METHODSExpression and promoter methylation of miR-720 were determined by quantitive PCR and pyrosequencing in 38 patients with AML and 20 normal controls. Lentivirous-mediated miR-702 overexpression was constructed in AML cell line kasumi-1. The cell proliferation, apoptosis, cycle, colony formation, migration and P53-mediated apoptosis pathway were determined.
RESULTSAML patients showed significantly lower miR-720 expression compared with normal controls (0.69±0.09 vs 3.00±0.46, P<0.01); The methylation level of miR-720 promoter region in AML patients were significantly higher than normal controls [(75.56±2.35)% vs (47.65±2.78)%, P<0.01]. miR-720 overexpression in kasumi-1 cells induced significantly increased cell apoptosis (P=0.017), elevated apoptosis sensitivity to etoposide (P=0.004), and reduced cell proliferation (P<0.01). miR-720 overexpression also induced reduced colony formation (P=0.005), cell cycle arrest in G(1)/G(0) phase and decreased migration ability in kasumi-1 cells. In addition, overexpression of miR-720 significantly induced increased cell apoptosis-related proteins including P53 and Bax, and activation of NF-κB signal transduction pathway. After kasumi-1 cells were treated with 1uM decitabine for 48 hours, miR-720 promoter methylation reduced significantly, and miR-720 expression significantly increased.
CONCLUSIONThe expression of miR-720 in AML patients reduced significantly, and DNA methylation-mediated epigenetic silencing of miR-720 contributed to maintain the malignant characteristics of AML.