Antiproliferative effect of silencing mTOR gene on MCL Jeko-1 cell line and its mechanism.
- VernacularTitle:shRNA干扰mTOR基因对套细胞淋巴瘤Jeko-1细胞增殖抑制及其作用机制研究
- Author:
Ruiji ZHENG
1
;
Yiqun HUANG
1
;
Xudong MA
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; Caspase 9; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Humans; Lymphoma, Mantle-Cell; RNA Interference; RNA, Messenger; RNA, Small Interfering; Signal Transduction; TOR Serine-Threonine Kinases; Transfection
- From: Chinese Journal of Hematology 2015;36(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of silencing mTOR gene by RNA interference on proliferation and apoptosis, and its mechanism on mantle cell lymphoma Jeko-1 cell Line.
METHODSThe hairpin-like oligonucleotide sequences targeting mTOR gene was designed and transfected into Jeko-1 cells by lipofectamine TM 2000. The mTOR mRNA and protein were detected by RQ-PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expressions of Bcl-2, Bax, procaspase-3, procaspase-9, P70S6K,and p-P70S6K were detected by Western blot.
RESULTSmTOR mRNA was markedly suppressed by shRNA targeting mTOR. mTOR shRNA suppressed proliferation and induced cells apoptosis of Jeko-1 cells. The cell apoptotic rates were (36.62 ± 3.24)%, (2.58 ± 1.04)%, (1.24 ± 0.30)% respectively, in mTOR shRNA, Neg-shRNA and Blank with statistically significant difference among them (P<0.05). mTOR shRNA down-regulated the expressions of Bcl-2, proCaspase3, proCaspase9 and p-70S6K, up-regulated the expression of Bax.
CONCLUSIONDeplete of mTOR gene may be realized through inhibiting the Akt/mTOR signaling pathway to promote the cell apoptosis and inhibit cell growth in Jeko-1 cell line.
