Expression of lung resistance protein and multidrug resistance protein genes in bone marrow cells of acute leukemia patients and its clinical significance.
- Author:
Zuo-Hua CHI
1
;
Zhen LIU
;
Chen SUN
;
Hong-Guo ZHAO
;
Jin-Lan LIU
Author Information
1. Institute of Hematology, Jinan University Medical College, Guangzhou 510630, China. chizuohua@hotmail.com
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Sub-Family B;
genetics;
Adolescent;
Adult;
Aged;
Bone Marrow Cells;
metabolism;
Female;
Humans;
Leukemia, Myeloid, Acute;
drug therapy;
metabolism;
Male;
Middle Aged;
Neoplasm Proteins;
genetics;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
drug therapy;
metabolism;
Prognosis;
RNA, Messenger;
analysis;
Vault Ribonucleoprotein Particles;
genetics
- From:
Journal of Experimental Hematology
2003;11(5):472-475
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of lung resistance protein (LRP) and multidrug resistance protein (MRP) genes in bone marrow cells in patients with acute leukemia and its clinical significance, expression of LRP and MRP mRNA in bone marrow cells from 47 cases of acute leukemia, including 10 refractory or relapsed cases, and 7 normal individuals were determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The result s showed that expression of LRP gene was negative in normal individuals. LRP mRNA level in newly treated cases of acute myelocytic leukemia and refractory or relapsed cases was significantly higher than that in normal individuals, increased LRP mRNA level has correlation with lower sensitivity to initial chemotherapy and was associated with reduced overall survival rate. Complete remission (CR) rate in LRP positive patients was lower than that in negative cases. The level of LRP expression was correlated with that of MRP mRNA. In conclusion, the expression of LRP mRNA can predict the treatment outcome and prognosis for acute myelocytic leukemia, prognosis was even worse in LRP and MRP linked expression cases, therefore, LRP was an important resistant factor, determination of LRP and MRP expression can help us to evaluate the prognosis and choose chemotherapy program.
