Comparison of the clonal expansion of TCR Vbeta T cells in patients with acute promyelocytic leukemia in vivo and in vitro.
- Author:
Li-Jian YANG
1
;
Yang-Qiu LI
;
Shao-Hua CHEN
;
Su-Fang HAN
;
Sheng-Ting CHEN
;
Xue-Li ZHANG
;
Tao ZHANG
Author Information
1. Institute of Hematology, Jinan University Medical College, Guangzhou 510632, China. jnyanglijian@163.net
- Publication Type:Journal Article
- MeSH:
Humans;
Leukemia, Promyelocytic, Acute;
genetics;
immunology;
Lymphocyte Activation;
Receptors, Antigen, T-Cell, alpha-beta;
genetics;
T-Lymphocytes;
immunology
- From:
Journal of Experimental Hematology
2003;11(5):499-502
- CountryChina
- Language:Chinese
-
Abstract:
In order to analyze the distribution and clonal expansion of TCR Vbeta subfamily T cells in patients with acute promyelocytic leukemia (APL) in vivo and in vitro after T cell culture, the peripheral blood mononuclear cells from 3 APL patients were expanded by rhIL-2 and anti-CD3 antibody using liquid T lymphocytes culture technique. The complementary determining region 3 (CDR3) of TCR beta with variable region genes was amplified in T cells from 3 APL cases before and after T cell culture by using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by genescan. The results showed that only a part of 24 Vbeta subfamilies was detected in T cells from the patients, and some Vbeta subfamily T cells could be identified after T cells culture. The clonal expansion T cells in some TCR Vbeta subfamilies could be found in all patients. The similar oligoclonal expansion of Vbeta1, Vbeta3, Vbeta7, Vbeta16 and Vbeta20 T cells was detected in two cases at different time points after T cell culture. It is concluded that the restricted expression of TCR Vbeta subfamily in T cells from patients might be the common feature in leukemia. Some Vbeta subfamily T cells could be induced after T cells culture in vitro. The continual clonal expansion of TCR Vbeta subfamily T cells at different time points after T cells culture could be a specific immune response of patients T cells related to the specific APL cell associated antigen.
