FasL-cDNA transfected into mouse bone marrow cells ex vivo to prevent graft versus host disease.
- Author:
Zhi-Liang XU
1
;
Ping ZOU
;
Ling-Bo LIU
;
Ai-Xiang LI
;
Yan-Ping MA
Author Information
1. Department of Pediatrics, People's Hospital, Wuhan University, Wuhan 430060, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
metabolism;
Bone Marrow Purging;
Bone Marrow Transplantation;
Fas Ligand Protein;
Female;
Genetic Therapy;
Graft vs Host Disease;
Male;
Membrane Glycoproteins;
genetics;
Mice;
Mice, Inbred BALB C;
Transfection
- From:
Journal of Experimental Hematology
2003;11(5):512-515
- CountryChina
- Language:Chinese
-
Abstract:
To explore the new approach to prevent graft versus host disease (GVHD) by purging ex vivo T lymphocytes of bone marrow graft through Fas-FasL way, FasL-cDNA was transfected into BALB/c mouse bon e marrow cells by liposome ex vivo. The transfected cells were cultured together with BAC (BALB/c x C57BL/6) mouse bone marrow graft. The mixing bone marrow graft was infused into BALB/c mouse recipients after 60Co-gamma irradiation. The mortality, manifestation and pathologic change of GVHD in recipient mice were observed. The CFU-S and Y chromosome from donor mice were detected. The results showed that compared with control group, the mortality in 60 days of the recipients in the experimental group decreased (20% vs 70%, P < 0.01) and the morbidity of GVHD lowered (40% vs 100%, P < 0.01). The CFU-S counts for all groups were at normal level on 20 days after transplantation. The Y chromosome from donor mice was discovered in 70% bone marrow nucleated cells of recipient mice survived over 2 months in the experimental group. It is concluded that mFasL-cDNA transfected mouse bone marrow cells prevent GVHD after culturing together with bone marrow graft, and accelerate hematopoietic reconstitution in recipient mice.
