Real-time quantitative PCR detecting minimal residual disease in multiple myeloma patients after autologous peripheral blood stem cell transplantation.
- Author:
Fa-Ying LIN
1
;
Jian HOU
;
Long-Yi TAN
;
Si-Qi DING
;
Hao WANG
;
Dong-Xing WANG
Author Information
1. Department of Hematology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China. linfaying@medmail.com.cn
- Publication Type:Journal Article
- MeSH:
Gene Rearrangement;
Humans;
Immunoglobulin Heavy Chains;
genetics;
Multiple Myeloma;
diagnosis;
genetics;
therapy;
Neoplasm, Residual;
Peripheral Blood Stem Cell Transplantation;
Polymerase Chain Reaction;
methods;
Sensitivity and Specificity;
Transplantation, Autologous
- From:
Journal of Experimental Hematology
2003;11(5):516-520
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the role of real-time PCR in detecting minimal residual disease in multiple myeloma and Waldenstrom's macroglobulinemia after autologous peripheral blood stem cell transplantation (APBSCT), real-time PCR was used to quantitate the IgH rearrangement in 8 patients with multiple myeloma (MM) and 1 case of Waldenstrom's macroglobulinemia before and after APBSCT. The results showed that the copies of IgH rearrangement pre- or post-APBSCT were 3108 +/- 1043 and 549 +/- 660 (P < 0.05) respectively. The number of IgH copies was positively correlated with the amount of plasmocytes in patient 's bone marrow and the M-protein in peripheral blood (r = 0.86, P < 0.05). Similar result was obtained in a case of relapsed Waldenstrom's macroglobulinemia. In conclusion, the quantitative analysis of IgH rearrangement by real-time PCR is a novel way to evaluate the therapeutic efficaciousness and predict the prognoses in MM patients.
