Human placenta derived adherent cells support in vitro expansion of umbilical cord blood CD34+ cells.
- Author:
Yi ZHANG
1
;
Jin HE
;
Xiao-Xia JIANG
;
Gang LIU
;
Yuan-Lin LIU
;
Pei-Hsien TANG
;
Ning MAO
Author Information
1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. zhangyi@nic.bmi.ac.cn
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Cell Adhesion;
Cell Separation;
Fetal Blood;
cytology;
Hematopoietic Stem Cells;
cytology;
Humans;
Placenta;
cytology
- From:
Journal of Experimental Hematology
2003;11(6):560-564
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to elucidate the effect of human placenta adherent cells (hPDAC) on expansion of human umbilical cord blood CD34(+) cells in vitro. hPDAC was isolated and characterized in human placenta tissue by using enzyme-digesting method and flow cytometry. A co-culture system was established with hPDAC and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of hPDAC and SCF, IL-3, IL-6 and FL. The number of total nucleated cells, CFC and CD34(+) cells were repeatedly counted in culture for 4 weeks. The results showed that the hPDAC displayed fibroblast-like morphology, and were positive for CD29, CD44, CD166, HLA-ABC and UEA-1, and negative for CD34, CD45 and HLA-DR. Functionally, ex vivo expansion of CD34(+) cells on feeder layer of placental adherent cells was significantly higher than that no feeder layer group. SCF + IL-3 + IL-6 + FL + hPDAC manifested the most potent combination, with the number of total nucleated cells increasing by (126.0 +/- 6.7)-fold, progenitor cells (CFC) (49.8 +/- 1.7)-fold and CD34(+) cells (8.3 +/- 1.65)-fold. It is concluded that placental adherent cells could support hematopoiesis in vitro and work as a suitable feeder layer for cord blood stem/progenitor cell expansion.
