The growth characteristics of mesenchymal stem/progenitor cells in human umbilical cord blood.
- Author:
Fan-Jun CHENG
1
;
Ping ZOU
;
Zhao-Dong ZHONG
;
Rong GUO
;
Juan XIAO
Author Information
1. Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Cell Adhesion;
Cell Division;
Fetal Blood;
cytology;
Humans;
Mesenchymal Stromal Cells;
physiology;
Tissue Engineering
- From:
Journal of Experimental Hematology
2003;11(6):565-568
- CountryChina
- Language:Chinese
-
Abstract:
This study was done for investigating the frequency and proliferative feature of mesenchymal stem/progenitor cells (MSPC) in human umbilical cord blood (CB) and for searching a new seed cell for tissue engineering. Mononuclear cells was separated by Ficoll-Hypaque from cord blood and suspended in DMEM culture medium supplemented by 2% fetal bovine serum. The adherent CB cells were cultured and expanded at same medium. The results showed that the frequency of CB-MSPC was 0.5 x 10(-6) [(0.2 - 0.8) x 10(-6)]. The CB-MSPC showed a fibroblast-like morphology and retained their morphological feature at least after 20 sub-passages, and could extensively be expanded by about 1.3 x 10(7) times as much. The conclusion is that low serum DMEM culture could maintain the proliferation and differentiation potential of CB-MSPC. CB-MSPC might be a favorable seed cell for tissue engineering and regeneration.
