Transduction efficiency of recombinant adeno-associated virus 2 in human umbilical cord blood CD34+ hematopoietic stem/progenitor cells.
- Author:
Yan CHEN
1
;
Fang-Ping CHEN
;
Jian-Qiang PENG
;
Xiao-Bing WU
;
Xin-Hua WU
;
Gan FU
Author Information
1. Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Dependovirus;
genetics;
Fetal Blood;
cytology;
Genetic Therapy;
Green Fluorescent Proteins;
Hematopoietic Stem Cells;
metabolism;
Humans;
Luminescent Proteins;
genetics;
Recombination, Genetic;
Transduction, Genetic
- From:
Journal of Experimental Hematology
2003;11(6):576-578
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the transduction efficiency of recombinant adeno-associated virus 2 (rAAV-2) in human umbilical cord blood CD34(+) hematopoietic stem/progenitor cells, the CD34(+) cells sorted by the method of magnetic cell sorting from human cord blood were infected with the rAAV-2 expressing the green fluorescent protein (GFP) gene. After transduction for 19 hours, the expression of GFP was detected under fluorescence microscope. The results showed that 43% CD34(+) cells expressed the GFP gene at a multiplicity of infection of 2 x 10(5). It is concluded that the rAAV-2 can transduce human cord blood CD34(+) hematopoietic stem/progenitor cells efficiently.
