Camouflage of HLA-I antigen in lymphocyte surface.
- Author:
Yin-Ze ZHANG
1
;
Wei LI
;
Hua-You ZHOU
;
Rong XIA
;
Jong-Cai LAN
;
Yang-Pei ZHANG
;
Zhi-Xin ZHANG
Author Information
1. Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. zyz200157@sohu.com
- Publication Type:Journal Article
- MeSH:
Antigen-Antibody Reactions;
Cytotoxicity, Immunologic;
Histocompatibility Antigens Class I;
immunology;
Humans;
Lymphocytes;
immunology;
Polyethylene Glycols;
pharmacology;
Triazoles;
pharmacology
- From:
Journal of Experimental Hematology
2003;11(6):622-624
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the method and effect of blocking the specific reaction between lymphocyte HLA-I antigen and its antibody. The lymphocytes were disposed with 12 mmol/L methoxypolyethelene glycol benzotriazol carbonate (mPEG-BTC) in concentration gradient in PBS (pH 7.4) at 22 degrees C. The effect of the modified lymphocytes was detected by microlymphocytotoxicity assay. The results showed that lymphocytes modified by mPEG-BTC did not react with related HLA-I antibodies in microcytotoxicity test. It is suggested that the specific reaction between HLA-I antigen of lymphocyte and HLA-I antibodies can be completely camouflaged by mPEG-BTC in PBS (pH 7.4) under 22 degrees C room temperature.
