Observation on gene polymorphism of Rh blood group in Chinese Han nationality.
- Author:
Jiong-Cai LAN
1
;
Cong-Rong WANG
;
Ya-Ming WEI
;
Hua-You ZHOU
;
Qiong CAO
;
Yin-Ze ZHANG
;
KuReXi JIANG
;
Da-Lin WU
;
Zhong LIU
Author Information
1. Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. DDiD21CN.com
- Publication Type:Journal Article
- MeSH:
Asian Continental Ancestry Group;
genetics;
China;
ethnology;
Humans;
Introns;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Rh-Hr Blood-Group System;
genetics
- From:
Journal of Experimental Hematology
2003;11(6):642-645
- CountryChina
- Language:Chinese
-
Abstract:
To observe the gene polymorphism of Rh blood group in unrelated random individuals and families for Chinese Han nationality, polymerase chain reaction-sequence specific primer (PCR-SSP) was used to amplify the Rh C/E gene, RhD gene, exons, intron 2 and 10, insert and Rh Box in 160 blood samples of RhD positive unrelated individuals and 71 samples of RhD negative unrelated individuals and 7 samples of families whose probands were RhD-negative. The results showed that RhD genes of RhD-negative individuals with C antigens were polymorphism, three forms were found for D exon including intact, partial deletion and complete deletion exons. Insert fragments and Rh Box were found in most cases of families whose probands were RhD-negative and its inheritance accorded with the Mendel's Law, and it did not affect the expression of RhD gene. "Normal" RhD exon 4 amplifying product was not found in all of the samples. It was concluded that gene structure of the RhD-negative in Chinese was polymorphism, intact, partial deletion and complete deletion exons were found in the individuals with C antigen and probably existed specific D (nf) Ce haplotype. The function of insert was uncertain. The Rh gene sequences of Chinese Han nationality are different from those of Caucasian and the Rh gene library based on Han nationality should be established.
