Up-regulation of transcription factors NF-E2, c-jun and c-fos of AP-1 family induced by Panax Notoginosides in hematopoietic cells.
- Author:
Rui-Lan GAO
1
;
Wei-Hong XU
;
Xiao-Hong CHEN
;
Xu-Dai QIAN
;
Chao-Qun WU
Author Information
1. Affiliated Hospital, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310006, China. zphtcmcn@mail.hz.zj.cn
- Publication Type:Journal Article
- MeSH:
DNA;
metabolism;
DNA-Binding Proteins;
genetics;
Erythroid-Specific DNA-Binding Factors;
Gene Expression Regulation;
drug effects;
Genes, fos;
Genes, jun;
Ginsenosides;
pharmacology;
HL-60 Cells;
Humans;
K562 Cells;
NF-E2 Transcription Factor;
NF-E2 Transcription Factor, p45 Subunit;
Panax;
Transcription Factor AP-1;
metabolism;
Transcription Factors;
genetics;
Up-Regulation
- From:
Journal of Experimental Hematology
2004;12(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effects of Panax Notoginosides (PNS) on up-regulation of AP-1 family transcription factors NF-E2, c-jun and c-fos for exploring intracellular signal pathway of PNS in hematopoietic cells, four human hematopoietic cells lines including myeloid HL-60, erythroid K562, megakaryoid CHRF-288 and Meg-01 were incubated in the presence of PNS for 14 days. The nuclear protein of cells were extracted and analyzed by Western blot with antibodies against NF-E2, c-fos and c-jun. Electrophoretic mobility shift assay (EMSA) was performed by using (32)P labeled AP-1 consensus oligonucleotide which contains binding site for NF-E2, c-jun and c-fos. The results showed that the transcription factors NF-E2, c-jun and c-fos of AP-1 family could be induced by PNS. Western blot demonstrated that the nuclear protein of both NF-E2 and c-jun in four cell lines treated by PNS were increased by 1.5-2.5- and 2.0-3.0-fold over untreated cells respectively. The c-fos protein in three cell lines of K562, CHRF-288 and Meg-01 was also elevated by 2.0-3.0-fold respectively, while c-fos protein in HL-60 cells was no detectable difference after PNS treatment. EMSA results in four cell lines indicated that AP-1 binding activity initiated by PNS was apparently elevated to form higher density band of AP-1-DNA complex. In conclusion, the intracellular transcription regulation initiated by PNS was involved in transcription factors NF-E2, c-jun and c-fos of AP-1 family members, which could play an important role in the up-regulation of genes expression related to proliferation and differentiation of hematopoietic cells.
