The effects of interferon-gamma on the expression of the cyclin D isoforms in cord blood hematopoietic stem/progenitor cells.
- Author:
Fan-Kai MENG
1
;
Xi-You TAN
;
Wen-Li LIU
;
Han-Ying SUN
;
Jian-Feng ZHOU
;
Yin-Li ZHOU
;
Ning WU
;
Lan SUN
Author Information
1. Department of Hematology, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
- Publication Type:Journal Article
- MeSH:
Cyclin D;
Cyclins;
genetics;
Fetal Blood;
cytology;
G1 Phase;
Hematopoietic Stem Cells;
drug effects;
metabolism;
Humans;
Interferon-gamma;
pharmacology;
Protein Isoforms;
RNA, Messenger;
analysis
- From:
Journal of Experimental Hematology
2004;12(2):138-141
- CountryChina
- Language:English
-
Abstract:
To explore the hematopoiesis inhibition mechanisms of interferon-gamma (IFN-gamma), the effects of IFN-gamma on the expression of the cyclin D in the umbilical cord blood hematopoietic stem/progenitor cells were observed. In the experiments the CD34(+) cells were isolated from the cord blood with MIDI-MACS system; semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; the expression levels of cyclin D isoforms were assayed by semi-quantitative RT-PCR, after the hematopoietic stem/progenitor cells were incubated with IFN-gamma. The results indicated that IFN-gamma could inhibit the formation of CFU-GM and down-regulate the expression of cyclin D2 and cyclin D3 at the mRNA level. It is concluded that the IFN-gamma could inhibit the proliferation of hematopoietic stem cells and down-regulate the expression of cyclin D, that may be one mechanism underlying the hematopoietic inhibition of IFN-gamma.
