Inhibiting effect of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotides on VEGF expression in U937 cell.
- Author:
Yun-Jie TONG
1
;
Min ZHANG
;
Ping ZOU
;
Rong GUO
Author Information
1. Department of Hematology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Oligodeoxyribonucleotides, Antisense;
pharmacology;
RNA, Messenger;
analysis;
U937 Cells;
Vascular Endothelial Growth Factor A;
analysis;
antagonists & inhibitors;
genetics
- From:
Journal of Experimental Hematology
2004;12(2):151-153
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of VEGF fully phosporothioated antisense oligodeoxynucleotide (VEGF-ASODN) on VEGF expression in acute monocyte leukemic cell line U937 in vitro, U937 cells were incubated with VEGF-ASODN at concentrations of 10, 20 and 30 micro mol/L or scrambled sequence as compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR. The expression of VEGF protein was measured by Western blot. The result showed that VEGF-ASODN had obviously inhibitive effect on expression of VEGF in U937 cell, as compared with scrambled sequence and negative control (P < 0.05). Scrambled sequence group had no significant difference compared with negative control group (P > 0.05). It is concluded that the expressions of VEGF mRNA and protein in leukemic cell line U937 are down-regulated by VEGF-ASODN.
