Inhibiting effects of stroma cell drived factor 1 (SDF-1) on proliferation of human acute myelocytic leukemia cell HL-60.
- Author:
Li WEI
1
;
Pei-Yan KONG
;
Xing-Hua CHEN
;
Xian-Gui PENG
;
Cheng CHANG
;
Dong-Feng ZENG
;
Hong LIU
;
Lin LIU
;
Qing-Yu WANG
;
Yi ZHANG
Author Information
1. Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
pharmacology;
Calcium;
metabolism;
Cell Cycle;
Cell Division;
Cell Survival;
Chemokine CXCL12;
Chemokines, CXC;
antagonists & inhibitors;
physiology;
HL-60 Cells;
cytology;
Humans;
Receptors, CXCR4;
analysis
- From:
Journal of Experimental Hematology
2004;12(2):154-158
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the importance of chemokine SDF-1 in maintaining proliferation ability of acute myelocytic leukemia cell line HL-60 when the effects of SDF-1 on HL-60 cell proliferation were inhibited. Marrow stromal cells were cultured and co-cultured with HL-60 cells, and SDF-1 activity was blocked with anti-CXCR4 McAb. HL-60 cell activity was detected by MTT while cell cycle and the expression of CXCR4 on HL-60 cell membrane were observed by flow cytometry meanwhile. The internal calcium ionic concentration in HL-60 cell was detected as well before and after treated with 12G5. The results showed that 12G5 down-regulated the expression of CXCR4 on HL-60 cell membrane; HL-60 cells at G(0)/G(1) phase increased, but decreased at S phase; survive rate of leukemia cells reduced; the intercellular calcium ionic concentration of HL-60 cell decreased after treated with 12G5. It was concluded that brockage of the SDF-1 activity may inhibit proliferation of leukemia cell at certain level.
