Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin.
- Author:
Xi ZHANG
1
;
Ping WANG
;
Xing-Hua CHEN
;
Lin LIU
;
Xian-Gui PENG
;
Qing-Yu WANG
;
Pei-Yan KONG
;
Hong LIU
;
Yi ZHANG
;
Lei GAO
;
Yong-Ming ZHONG
Author Information
1. Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
physiology;
Cell Survival;
drug effects;
Coculture Techniques;
Dose-Response Relationship, Drug;
Drug Resistance, Neoplasm;
HL-60 Cells;
drug effects;
Humans;
Idarubicin;
pharmacology;
Stromal Cells;
physiology
- From:
Journal of Experimental Hematology
2004;12(2):163-165
- CountryChina
- Language:Chinese
-
Abstract:
To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
