OBJECTIVETo investigate the effects of sinomenine on growth and apoptosis of MCL Jeko-1 cell line and its mechanism.

METHODSThe proliferation rate of Jeko-1 cells treated by different doses of sinomenine was assayed by MTT method; and the cell apoptosis was detected by flow cytometry. The expressions of Cyclin D1, BCL-2, BAX, Caspase-3, Akt signaling pathway protein Akt, phosphorylated-Akt (p-Akt), and phosphorylated-mTOR (p-mTOR), phosphorylated- P70S6K(p-P70S6K) were determined by Western blot.

RESULTSThe growth of Jeko-1 cell line was inhibited by Sinomenine. The apoptosis rates of Jeko-1 cells treated by 0, 1, 2, and 4 mmol /L of Sinomenine for 24 hours were (2.21±1.05) %, (11. 29±2.42)%, (18.79±2.84) %, (31.05±3.52) % respectively, and with very statistically significant difference(P<0.01). The expressions of p-Akt, p-mTOR, p-P70S6K were down-regulated, but total Akt expression was not changed. The expressions of cyclin D1 and BCL-2 were down-regulated, but that of BAX, and Caspase-3 were up-regulated.

CONCLUSIONThe sinomenine can inhibit Jeko-1 cell proliferation, which may be realized through down-regulating the phosphorylation level of p-Akt, p-mTOR, and p-P70S6K, thus inhibiting the Akt signaling pathway and promoting the cell apoptosis.