- Author:
Wei-Jiang LIU
1
,
2
;
Xue LI
3
;
Yuan-Lin LIU
3
;
Peng WANG
3
;
Yue FAN
3
;
Tian-Yi TENG
3
;
Bo-Qian BAI
4
;
Yue TONG
5
;
Wei ZHANG
3
;
Yi ZHANG
1
;
Author Information
- Publication Type:Journal Article
- From: Journal of Experimental Hematology 2018;26(1):251-257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of MicroRNA-3963(miR-3963) on the adipogenic differentiation of mouse bone-derived mesenchymal stem cells(MSC).
METHODSMSCs were isolated from C57BL/6 mice bone fragment and transfected with miR-3963 mimic, miR-3963 inhibitor and negative control. The expression of miR-3963 and transfection efficiency were detected by q-PCR. These transfected cells were induced to adipocytes and stained with oil red O after 14 days culture. q-PCR and Western blot were used to detect the expression of adipogenic differentiation marker genes C/EBPα and PPARγ at transcriptional level and protein level.
RESULTSThe results of q-PCR revealed that miR-3963 expression level was up-regulated after transfection with miR-3963 mimic (P<0.0001), and down-regulated after transfection with miR-3963 inhibitor (P<0.0001). After oil red staining, overexpression of miR-3963 in MSCs could promote the formation of lipid droplet. The q-PCR and Western blot analyses showed the significant increase of expression of adipogenic marker genes C/EBPα and PPARγ in MSC transfected with miR-3963 mimic. Additionally, compared with the control group, miR-3963 inhibitor could decrease adipogenic differentiation of MSC.
CONCLUSIONmiR-3963 can regulate and promote adipogenic differentiation of mouse bone-derived MSC.