Impact of glycogen synthase kinase-3β (GSK-3β) inhibitor on Wnt and NF-κB signal pathways in a rat model of diabetic nephropathy.
- Author:
Yixia ZHOU
1
;
Long LI
;
Yanni YU
2
;
Hua YANG
;
Huan YAO
;
Yang ZHANG
;
Li ZHANG
;
Chaonan DENG
;
Lina ZHAO
;
Ying ZHANG
;
Bin SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diabetic Nephropathies; metabolism; Disease Models, Animal; Glycogen Synthase Kinase 3; antagonists & inhibitors; metabolism; Glycogen Synthase Kinase 3 beta; Kidney; pathology; NF-kappa B; metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; Wnt Signaling Pathway
- From: Chinese Journal of Pathology 2015;44(11):783-787
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the impact of glycogen synthase kinase-3β (GSK-3β) on Wnt and NF-κB pathways in a rat model of diabetic nephropathy (DN).
METHODSSD rats were randomly divided into normal control group (NC), DN model group (DM) and GSK-3β inhibitor group (DI). Blood glucose and 24-hour urine protein were monitored in three groups. Renal tissue samples were stained by HE. The expression of GSK-3β and NF-κB proteins was studied by immunohistochemistry. GSK-3β and NF-κB mRNAs were detected by RT-qPCR.
RESULTSTen weeks after STZ injection, the level of blood glucose increased significantly in DM group [(23.2±5.4) mmol/L] and DI group [(25.0±4.0) mmol/L], compared with NC group, and the level of 24-hour urinary protein increased significantly in DM group [(185.2±35.6) g/24 h] and DI group [(179.6±44.7) g/24 h], compared with NC group. Two weeks after LiCl injection, the level of blood glucose and 24-hour urinary protein decreased in DI group (17.6±2.1) mmol/L, (106.9±30.0) g/24 h], compared with DM Group. Compared with NC group, pathological changes of the kidney of DM group aggravated along with increased mRNA and protein expression of GSK-3β and NF-κB. But the pathological changes of the kidney in DI group alleviated along with declined mRNA and protein expression of GSK-3β and NF-κB as compared with DM group (all P<0.05).
CONCLUSIONSNF-κB protein expression positively correlates with the GSK3β expression. Wnt and NF-κB signal pathways play an important role in the development of diabetic nephropathy.