Influence of polypeptide extract from scorpion venom on PI3K and p-Akt signaling protein expression and cell proliferation of K562 cells.
- Author:
Wen-Jun YU
1
;
Wen-Hua YANG
;
Xiang-Dong YANG
;
Zhe-Xin SHI
;
Xing-Li WANG
;
Zheng HAO
;
Jia ZHANG
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Humans;
K562 Cells;
Phosphatidylinositol 3-Kinases;
metabolism;
Proto-Oncogene Proteins c-akt;
metabolism;
Scorpion Venoms;
pharmacology;
Signal Transduction;
drug effects
- From:
Journal of Experimental Hematology
2012;20(4):872-875
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of polypeptide extract from scorpion venom (PESV) on PI3K, p-Akt signal protein regulating K562 cell apoptosis and its mechanism. The K562 cells were cultured with PESV for different time, the cell growth curve was determined by MTT method, the levels of PI3K and p-Akt proteins were detected by Western blot. The results showed that as compared with control group, the apoptosis rate of K562 cells treated with PESV increased, the levels of PI3K and p-Akt expression decreased. It is concluded that the PESV inhibits the proliferation and promotes the apoptosis of K562 cells probably through suppressing the expression of PI3K and p-Akt signal proteins.
