Effect of astragalus polysaccharide on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism.
- Author:
Peng-Yun ZENG
1
;
Li-Li DENG
;
Ling-Ling YUE
;
Lian-Sheng ZHANG
Author Information
1. Department of Hematology, Second Hospital of Lanzhou University, Lanzhou, China.
- Publication Type:Journal Article
- MeSH:
Astragalus Plant;
Cytotoxicity, Immunologic;
drug effects;
HL-60 Cells;
Histocompatibility Antigens Class I;
metabolism;
Humans;
Killer Cells, Natural;
Polysaccharides;
pharmacology
- From:
Journal of Experimental Hematology
2012;20(4):880-883
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to explore the effect of astragalus polysaccharide (APS) on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism. The cytotoxicities of NK cells against HL-60 cells were analyzed by LDH releasing assay at different effect-to-target cell ratios (E:T) before and after treated with APS. The gene expression of MHC class I chain-related (MICA) in HL-60 cells before and after APS treatment was assayed with RT-PCR. Protein expression of MICA in HL-60 cells was assayed by flow cytometry before and after treated by APS. The results showed that after treated with APS 15 mg/ml for 48 h, the cytotoxicities of NK cells against HL-60 cells enhanced at different effect-to-target (P < 0.05), and the gene and protein expressions in MICA of HL-60 cells were up-regulated (P < 0.05). It is concluded that the APS can obviously up-regulate the expression of MICA in HL-60 cells, thus enhance sensitivity of HL-60 cells to cytotoxicity of NK cells.
